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Showing posts from May, 2023

Micro-pipetting Tips And Tricks

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What is a Micropipette? A micropipette is a laboratory tool used to dispense small and precise volumes of liquid in the range of microliters (µL) to milliliters (mL). It consists of a plunger, a priston, and a tip that can be easily attached and removed. Micropipettes are commonly used in various applications, such as molecular biology, biochemistry, analytical  chemistry, and medical diagnostics. They are essential for many laboratory procedures that require accuracy, precision, and reproducibility.   What is Micropipetting? Micropipetting is a fundamental technique used in many laboratories for accurate and precise transfer of small volumes of liquids. However, it can be challenging for new users to master.  Accumax is one of the fastest growing liquid handling equipment globally. It delivers quality pipettes - Fab Pipette , Accumax - Smart , Accumax A Series , Accumax PRO , Accumax VA-FA , Electronic Pipette , Junior Pipette , and Transfer Pipette . Choose the r...

Real Time PCR the most recognized Molecular Biology technique of 21st Century

As someone who has worked on this machine from the early 2000’s, I never would have imagined that post-2019 ‘Real Time PCR’ would become a household name, but that’s exactly what Real Time is. The world has changed forever after 2019 & this word Real time PCR would ever be etched in the memory of survivors; for some as its results were & are & will be a relief, for others its results give a sinking feeling & will continue to; but for all those who have run the experiments on real time let me say its an enriching experience as we come face to face with what our genes do, behave or what infections we have. The following section is a very thin informative tapestry of the technique which is often called the  ‘Queen of Gene Expression’. The invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 was the impetus in giving birth to real-time PCR in the Mid 1990s. Detection and expression analysis of genes in real-time has revolutionized the 21st-cent...