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Showing posts from April, 2023

THINGS TO REMEMBER WHILE USING BOTTLE TOP DISPENSERS

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  Bottle Top Dispensers A bottle top dispenser is a laboratory instrument used for dispensing precise volumes of liquids from bottles. It typically consists of a device that is attached to the top of a bottle, allowing for controlled and accurate dispensing of liquid samples.  Bottle top dispensers  are commonly used in laboratories for tasks such as measuring and dispensing reagents, chemicals, or other liquids in a controlled manner.   Bottle top dispensers often have a built-in mechanism that allows the user to set the desired volume to be dispensed. This can be done through a volume adjustment knob or a digital display. The dispenser typically includes a piston or a plunger that is used to draw liquid from the bottle and dispense it in a controlled manner. Some bottle top dispensers also come with interchangeable nozzles of various sizes to accommodate different bottle types and volumes.  Bottle Top Dispensers are widely used in various f...

Importance of certified lab equipment for Precision and Accuracy in Dispensing

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  As liquid handling is a core component in food, biochemical, analytical, environmental, and pharmaceutical industrial processes, standard measurement methods are necessary for validation of your assay and qualification of liquid handling devices used. Conceptualize that you are on an experiment with all necessary precautions along with required SOP and there is no scope for an error to happen. Here comes the importance of the equipment’s precision and accuracy being used to understand the deviation of the dispensed volumes from the target volume. For example, the lab equipment is supposed to dispense 20 or 200 microliters, but it has dispensed 22 or 220 microliters respectively, this shows the precision but this dispensing is not accurate. Thus, purchasing equipment certified through  ISO/IEC 17025 is very important to assure the metrological traceability of that equipment. The ISO 17025 accreditation provides the highest level of reliability and confidence in pipette calibr...

Tips to Improve Pipetting of Viscous solution

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  1. Methodology to be considered for air displacement pipetting If you are going with air displacement then do reverse pipetting. This technique results in the sample volume plus an additional volume being aspirated into the tip. It can be achieved by pressing to the first stop to expel only the target sample volume. Forward mode pipetting is not recommended, as it often leaves a residual volume of undelivered sample in the tip. 2. Use Appropriate pipette tip 3. Immerse the Tip to a Proper depth and hold vertically while Aspiration Immersion depth should be in track to the liquid volume 4. Reduce Plunger speed and pause sufficiently after aspiration and dispense The appropriate pause duration should be determined at the beginning and kept consistent each time. To determine whether the pause after aspiration is long enough, examine the filled tip after removing from solution. If a trailing air gap forms, a longer pause needed. After dispensing, examine the bottom of the pipette tip...

LOW RETENTION PIPETTE TIPS, EVERY DROP COUNTS

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Low retention tips, also known as low binding tips, are specially designed pipette tips that reduce the adhesion of various materials like DNA, enzymes, proteins, and cells to the tip surface. These tips are made using polymer technology that makes the inner surface of the pipette tip more hydrophobic, resulting in a significant reduction in sample loss due to adhesion. Compared to ordinary pipette tips, low-retention pipette tips reduce sample retention by three to five times. These tips are designed to improve sample accuracy, increase reliability, save expensive reagents, deliver samples precisely, and are 100% inert. Overall, low-retention pipette tips offer improved liquid handling and less liquid retention, making them a valuable tool in the laboratory. ACCUMAX LOW RETENTION TIPS IMPROVE PIPETTING Some liquids tend to adhere to the pipette tips and adversely affect the confidence interval of the assay, especially during pipetting application involving serial dilution  To ov...